rabbit anti mpc1 polyclonal antibodies (OriGene)
OriGene is a verified supplier 
OriGene manufactures this product 
90
Structured Review
OriGene
rabbit anti mpc1 polyclonal antibodies
Rabbit Anti Mpc1 Polyclonal Antibodies, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+mpc1+polyclonal+antibodies/BRP44L+(MPC1)+Rabbit+Polyclonal+Antibody/pm32066834-250-38-44
Average 90 stars, based on 2 article reviews
Rabbit Anti Mpc1 Polyclonal Antibodies, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+mpc1+polyclonal+antibodies/BRP44L+(MPC1)+Rabbit+Polyclonal+Antibody/pm32066834-250-38-44
Average 90 stars, based on 2 article reviews
rabbit anti mpc1 polyclonal antibodies - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes."
Article Title: Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes.
Journal: Scientific reports
doi: 10.1038/s41598-020-59709-6
Figure Legend Snippet: Figure 4. Effects of down regulating related genes in DOs or CCM on in vitro maturation of pig oocytes. Graph (A) shows percentages of MII oocytes after DOs were cultured for 48 h in the NCSU-23 medium containing 0, 10 or 15 mM pyruvate (Pyr) with 10, 25 or 50 µM 4-CIN (CIN) or with 0.01, 0.03 or 0.05 µM rotenone (Rot). Graph B shows percentages of MII oocytes after DOs were cultured for 48 h in the NCSU-23 medium containing 0, 5 or 10 mM lactate (Lac) with 10, 25 or 50 mM sodium oxamate (Oxm) or with 10, 20 or 50 µM 4-CIN. Graphs (C–E) show MII percentages of cocultured DOs (Left Y axis) and levels of MPC1, NDUFV1 and LDHB (Right Y axis) after transfection of CCM with negative control (NC) siRNA, MPC1 siRNA (MP1, 2 or 3), NDUFV1 siRNA (ND1, 2 or 3), or LDHB siRNA (LD1, 2 or 3), respectively. Graphs (F,G) show MII percentages of DOs after microinjection with NC, MP3, ND1 or LD2 siRNAs. While DOs and the CCM transfected/injected with MPC1 or NDUFV1 siRNAs were cultured in NCSU-23 containing 15 mM pyruvate, DOs and the CCM transfected/injected with LDHB siRNA were cultured in NCSU-23 containing 10 mM lactate. In oocyte maturation experiments, each treatment was repeated 4 times with each replicate containing about 20 oocytes. Graphs (H,I) show ATP concentrations (ng/ml) in medium conditioned with CCM in NCSU-23 medium containing 5.6 mM glucose and 15 mM pyruvate, respectively, after transfection with G6PD siRNA-3 (G6-3), GAPDH siRNA-3 (GA-3) or MPC1 siRNA-3 (MP3). Each treatment was repeated 3 times with each replicate containing medium recovered from one culture well on different experimental days. a–f: Values with a different letter above bars differ significantly (P < 0.05).
Techniques Used: In Vitro, Cell Culture, Transfection, Negative Control, Microinjection, Injection
Related Articles
Incubation:Article Title: Glucose metabolism during in vitro maturation of mouse oocytes: An study using RNA interference. Article Snippet: This article is protected by copyright.. All rights reserved In previous studies on glucose metabolism during in vitro maturation, intact cumulus-oocyte complexes (COCs) were treated with enzyme inhibitors/activators.. Because inhibitors/activators may have non-specificity and/or toxicity, and culture of COCs cannot differentiate whether glucose metabolism of cumulus cells (CCs) or that of the oocyte supports oocyte maturation, results from the previous studies must be verified by silencing genes in either CCs or cumulus-denuded oocytes (DOs). Bioprocessing:Article Title: Glucose metabolism during in vitro maturation of mouse oocytes: An study using RNA interference. Article Snippet: This article is protected by copyright.. All rights reserved In previous studies on glucose metabolism during in vitro maturation, intact cumulus-oocyte complexes (COCs) were treated with enzyme inhibitors/activators.. Because inhibitors/activators may have non-specificity and/or toxicity, and culture of COCs cannot differentiate whether glucose metabolism of cumulus cells (CCs) or that of the oocyte supports oocyte maturation, results from the previous studies must be verified by silencing genes in either CCs or cumulus-denuded oocytes (DOs). Article Title: Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes Article Snippet: Western blotting of CCs was performed as reported previously . (i) A radioimmuno-precipitation assay (RIPA) buffer was prepared, which contained 150 mM NaCl, 1.0% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM phenylmethyl sulfonyl fluoride, and 50 mM Tris (pH 8). (ii) CCs from 2 wells of monolayer culture in a 96-well plate were washed in cooled PBS, and lysed in 20 μl RIPA buffer. (iii) After the total protein concentration was determined using a BCA Protein Assay Kit (P0012; Beyotime Institute of Biotechnology) and adjusted to 1 μg/μl, 20 μl were placed in a 0.5-ml microfuge tube and frozen at −80 °C until use. (iv) 5 μl of 5 × sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) loading buffer were added to each tube, and the tubes were heated to 100 °C for 5 min to extract protein. (v) To separate total proteins, a SDS-PAGE was run on a 10% polyacrylamide gel and the proteins obtained were transferred electrophoretically onto polyvinylidene fluoride membranes. (vi) The membranes were washed in TBST (150 mM NaCl, 2 mM KCl, 25 mM Tris and 0.05% Tween 20; pH 7.4), blocked for 2 h at 37 °C with TBST containing 3% BSA, and incubated at 4 °C overnight with primary antibodies. (vii) After being washed in TBST, the membranes were incubated for 1.5 h at 37 °C with secondary antibodies. .. The primary antibodies used included rabbit anti-G6PD monoclonal antibodies (1:1000, ab993, Abcam Co., Ltd, Beijing, China), mouse anti-GAPDH monoclonal antibodies (1:1000, CW0100A, CWBio Co., Ltd, Beijing, China), rabbit anti-LDHB polyclonal antibodies (1:1000, 14824-1-AP, Proteintech Co., Ltd, Wuhan, China), Article Title: Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes. Article Snippet: Western blotting of CCs was performed as reported previously47. (i) A radioimmuno-precipitation assay (RIPA) buffer was prepared, which contained 150 mM NaCl, 1.0% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM phenylmethyl sulfonyl fluoride, and 50 mM Tris (pH 8). (ii) CCs from 2 wells of monolayer culture in a 96-well plate were washed in cooled PBS, and lysed in 20 μl RIPA buffer. (iii) After the total protein concentration was determined using a BCA Protein Assay Kit (P0012; Beyotime Institute of Biotechnology) and adjusted to 1 μg/μl, 20 μl were placed in a 0.5-ml microfuge tube and frozen at −80 °C until use. (iv) 5 μl of 5 × sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) loading buffer were added to each tube, and the tubes were heated to 100 °C for 5 min to extract protein. (v) To separate total proteins, a SDS-PAGE was run on a 10% polyacrylamide gel and the proteins obtained were transferred electrophoretically onto polyvinylidene fluoride membranes. (vi) The membranes were washed in TBST (150 mM NaCl, 2 mM KCl, 25 mM Tris and 0.05% Tween 20; pH 7.4), blocked for 2 h at 37 °C with TBST containing 3% BSA, and incubated at 4 °C overnight with primary antibodies. (vii) After being washed in TBST, the membranes were incubated for 1.5 h at 37 °C with secondary antibodies. .. The primary antibodies used included rabbit anti-G6PD monoclonal antibodies (1:1000, ab993, Abcam Co., Ltd, Beijing, China), mouse anti-GAPDH monoclonal antibodies (1:1000, CW0100A, CWBio Co., Ltd, Beijing, China), rabbit anti-LDHB polyclonal antibodies (1:1000, 14824-1-AP, Proteintech Co., Ltd, Wuhan, China), |
